Articolo in rivista, 2011, ENG, 10.3892/ijo.2011.988
Giovanna Barbieri, Emilio Rimini and Maria Assunta Costa.
Istituto di Biomedicina e Immunologia Molecolare 'Alberto Monroy', Consiglio Nazionale delle Ricerche (CNR), Palermo, Italy.
Melanoma cells often display constitutive expres-sion of the major histocompatibility complex (MHC) class II molecules which is associated with a higher metastatic dissemination. The MHC class II molecules during T cell/ professional antigen-presenting cell (APC) interactions are localized, as signalling receptors, into membrane lipid rafts which are thought to be sites of signalling complex assembly. Therefore, with the aim of understanding the molecular mechanisms used by melanoma cells to frustrate an effective anti-tumour response we stimulated a MHC class II constitutive expressing melanoma cell line with a specific antibody that mimics the interaction of T-cell receptor (TCR) with class II molecules. In stimulated melanoma cells we showed through Western blotting and immunofluorescence experiments the recruitment of HLA-DR molecules in lipid raft compartments as well as the c-Jun NH2-terminal kinase activations as a consequence of the class II engagement. Furthermore, we showed that SDS-stable HLA-DR-peptide complexes are recruited in lipid rafts of stimulated melanoma cells. Therefore, in light of the results reported, our hypothesis is that the redistribution of class II molecules into lipid raft microdomains of stimulated melanoma cells as well as the associated activation of signalling pathways, could be useful for melanoma cells to frustrate an effective anti-tumour response.
International journal of oncology 38 , pp. 1589–1595
Antibodies, Monoclonal; Cell Line, Tumor; HLA-DR Antigens; Humans; Melanoma; Membrane Microdomains; Protein Transport; Signal Transduction; Subcellular Fractions
Barbieri Giovanna, Costa Maria Assunta
IBIM – Istituto di biomedicina e di immunologia molecolare "Alberto Monroy"
ID: 12583
Year: 2011
Type: Articolo in rivista
Creation: 2011-05-17 00:00:00.000
Last update: 2021-01-29 17:42:14.000
CNR authors
External IDs
CNR OAI-PMH: oai:it.cnr:prodotti:12583
DOI: 10.3892/ijo.2011.988