Articolo in rivista, 2008,

Mammalian complex I: A regulable and vulnerable pacemaker in mitochondrial respiratory function

Sergio Papa, Domenico De Rasmo, Salvatore Scacco, Anna Signorile, Zuzana Technikova-Dobrova, Giuseppe Palmisano, Anna Maria Sardanelli, Francesco Papa, Damiano Panelli, Raffaella Scaringi and Arcangela Santeramo

Department of Medical Biochemistry, Biology and Physics (DIBIFIM), University of Bari, Italy Institute of Biomembranes and Bioenergetics (IBBE), Consiglio Nazionale delle Ricerche, Italy Department of Odontostomatology and Surgery, University of Bari, Bari, Italy Institute of Microbiology, Czech Academy of Sciences, Prague, Czech Republic

In this paper the regulatory features of complex I of mammalian and human mitochondria are reviewed. In a variety of mitotic cell-line cultures, activation in vivo of the cAMP cascade, or direct addition of cAMP, promotes the NADH–ubiquinone oxidoreductase activity of complex I and lower the cellular level of ROS. These effects of cAMP are found to be associated with PKA-mediated serine phosphorylation in the conserved C-terminus of the subunit of complex I encoded by the nuclear gene NDUFS4. PKA mediated phosphorylation of this Ser in the C-terminus of the protein promotes its mitochondrial import and maturation. Mass-spectrometry analysis of the phosphorylation pattern of complex I subunits is also reviewed.

Keywords

Complex I, Proton pump, cAMP cascade, PKA, Mitochondrial import

CNR authors

Papa Sergio

CNR institutes

IBIOM – Istituto di Biomembrane, Bioenergetica e Biotecnologie Molecolari

ID: 12845

Year: 2008

Type: Articolo in rivista

Creation: 2009-06-16 00:00:00.000

Last update: 2009-06-16 00:00:00.000

CNR authors

External links

OAI-PMH: Dublin Core

OAI-PMH: Mods

OAI-PMH: RDF

External IDs

CNR OAI-PMH: oai:it.cnr:prodotti:12845