Articolo in rivista, 2008, ENG, 10.1117/1.2830824

Three-Dimensional Real-Time Imaging of Cardiac Cell Motions in Living Embryos

Jian Lu (1); Francisco Pereira (2); Scott E. Fraser (3); Morteza Gharib (1)

(1) California Institute of Technology, Option of Bioengineering, Pasadena, California 91125; (2) INSEAN Italian Ship Model Basin, I-00128 Rome, Italy; (3) California Institute of Technology, Biological Imaging Center, Pasadena, California 91125.

While quantitative analysis of dynamic biological cell motions in vivo is of great biomedical interest, acquiring 3-D (plus time) information is difficult due to the lack of imaging tools with sufficient spatial and temporal resolution. A novel 3-D high-speed microscopic imaging system is developed to enable 3-D time series data acquisition, based on a defocusing technique (DDPIV). Depth coordinate Z is resolved by the triangular image patterns generated by a mask with three apertures forming an equilateral triangle. Application of this technique to microscale imaging is validated by calibration of targets spread over the image field. 1-?m fluorescent tracer particles are injected into the blood stream of 32h post-fertilization developing zebrafish embryos to help describe cardiac cell motions. 3-D and velocity fields of cardiovascular blood flow and trajectories of heart-wall motions are obtained.

Journal of biomedical optics 13 (1), pp. 014006-1–014006-8

Keywords

cardiac cell motions, DDPIV, tracking

CNR authors

Alves Pereira Francisco

CNR institutes

INM – Istituto di iNgegneria del Mare