Articolo in rivista, 2011, ENG, 10.1002/mabi.201000354

Poly(ester urethane) guides for peripheral nerve regeneration

Valeria Chiono, Susanna Sartori, Alfonsina Rechichi, Chiara Tonda-Turo, Giovanni Vozzi, Federico Vozzi, Mario D'Acunto, Claudia Salvadori, Francesca Dini, Giovanni Barsotti, Fabio Carlucci, Silvia Burchielli, Silvia Nicolino, Chiara Audisio, Isabelle Perroteau, Paolo Giusti, Gianluca Ciardelli

V. Chiono, S. Sartori, A. Rechichi, C. Tonda-Turo, G. Ciardelli - Department of Mechanics, Politecnico di Torino, Turin, Italy V. Chiono, S. Sartori, A. Rechichi, G. Vozzi, M. D'Acunto, P. Giusti, G. Ciardelli - Department of Chemical Engineering, Industrial Chemistry and Materials Science, University of Pisa, Pisa, Italy G. Vozzi, P. Giusti - C.N.R. Institute for Composite and Biomedical Materials, Pisa, Italy F. Vozzi - Centro ''E. Piaggio'', University of Pisa, Pisa, Italy F. Vozzi, S. Burchielli - C.N.R. Institute of Clinical Physiology, Pisa, Italy C. Salvadori - Department of Animal Pathology, Prophylaxis and Hygiene of Aliments, University of Pisa, Pisa, Italy F. Dini, G. Barsotti, F. Carlucci - Department of Veterinary Clinic, Faculty of Veterinary, University of Pisa, Pisa, Italy S. Nicolino, C. Audisio, I. Perroteau - Department of Animal and Human Biology, University of Turin, Torino, Italy

A biocompatible and elastomeric PU was synthesized from low-molecular-weight PCL as macrodiol, CMD as chain extender and HDI as chain linker for applications in the field of peripheral nerve repair. PU cast films supported in vitro attachment and proliferation of NOBEC. The in vitro adhesion and proliferation of S5Y5 neuroblastoma cells on the inner surface of uncoated, gelatin- and PL-coated PU guides were compared. Due to their superior in vitro performance, PL-coated PU guides were tested in vivo for the repair of 1.8 cm-long defects in rat sciatic nerves. The progressive regeneration was confirmed by EMG and histological analysis showing the presence of regenerating fibers in the distal stumps. Artificial nerve guides are a promising alternative to autografting for peripheral nerve repair. A newly synthesized biocompatible elastomeric poly(ester urethane) containing poly(?-caprolactone) segments was proposed for the realization of nerve guides. PU physicochemical and biological properties were analyzed. Successful nerve regeneration was demonstrated in a rat sciatic nerve model.

Macromolecular bioscience (Print) 11 (2), pp. 245–256

Keywords

CNR authors

Burchielli Silvia, Vozzi Federico, D Acunto Mario

CNR institutes

ISTI – Istituto di scienza e tecnologie dell'informazione "Alessandro Faedo"

ID: 199501

Year: 2011

Type: Articolo in rivista

Creation: 2013-01-21 12:55:43.000

Last update: 2013-05-30 13:05:54.000

External IDs

CNR OAI-PMH: oai:it.cnr:prodotti:199501

DOI: 10.1002/mabi.201000354

ISI Web of Science (WOS): 000287162800009

Scopus: 2-s2.0-79251542658