Articolo in rivista, 1997, ENG, 10.1016/S1011-1344(96)07475-1
Nicola Angelini and Rinaldo Cubeddu and Francesco Lenci and Aba Losi and Antonio Pifferi and Antonella Sgarbossa and Paola Taroni and Arnaldo Vecli and Cristiano Viappiani
Istituto Biofisica CNR, Via S. Lorenzo 26, 56127 Pisa, Italy CEQSE CNR e Dip. Fisica Politecnico, P. le Da Vinci, 20133 Milano, Italy Dipartimento Fisica Università, V. le Scienze, 43100 Porma, Italy Dipartimento Scienze Ambiente Territorio Università, Via A. Volta 4, 56100 Pisa, Italy
Liposomes loaded with hypericin at different local concentrations were used as artificial models of biological light sensors. The steady state and time-resolved fluorescence of the chromophore were investigated in the presence of fluorescence quenchers which penetrate differently into and diffuse differently through lipid bilayers: iodide (I-), 9,10-anthraquinone-2,6-disulphonate (AQDS2-) and 9,10-anthraquinone-2-sulphonate (ACMS-). The results of the experiments with I- indicate that two of the three fluorescent species detected in our system, the long-lived and intermediate-lived species, are relatively close to the lipid-water interface. Anthraquinone compounds, which partially penetrate into the liposome, not only significantly quench the chromophore fluorescence emission, but also, apparently, affect the distribution of hypericin molecules in the vesicle, shifting the long-lived fluorescent molecules towards chemical-physical configurations characterized by shorter fluorescence lifetimes.
Journal of photochemistry and photobiology. B, Biology (Print) 38 (2-3), pp. 245–252
Blepharisma japonicum, Photoreception, Photosensory transduction, Quenchers
Cubeddu Rinaldo, Pifferi Antonio Giovanni, Taroni Paola, Viappiani Cristiano, Sgarbossa Antonella, Angelini Nicola, Lenci Francesco
ID: 266837
Year: 1997
Type: Articolo in rivista
Creation: 2013-10-27 16:40:23.000
Last update: 2022-11-17 15:50:27.000
CNR institutes
External links
OAI-PMH: Dublin Core
OAI-PMH: Mods
OAI-PMH: RDF
DOI: 10.1016/S1011-1344(96)07475-1
URL: http://www.sciencedirect.com/science/article/pii/S1011134496074751
External IDs
CNR OAI-PMH: oai:it.cnr:prodotti:266837
DOI: 10.1016/S1011-1344(96)07475-1
ISI Web of Science (WOS): A1997XE03000025