Articolo in rivista, 2015, ENG, 10.1016/j.marenvres.2015.06.018

The response of Phaeodactylum tricornutum to quantum dot exposure: Acclimation and changes in protein expression.

Morelli E., Salvadori E., Basso B., Tognotti D., Cioni P., Gabellieri E.

CNR - Istituto di Biofisica

Nanotechnology has a great potential to improve life and environmental quality, however the fate of nanomaterials in the ecosystems, their bioavailability and potential toxicity on living organisms are still largely unknown, mainly in the marine environment. Genomics and proteomics are powerful tools for understanding molecular mechanisms triggered by nanoparticle exposure. In this work we investigated the effect of exposure to CdSe/ZnS quantum dots (QDs) in the marine diatom Phaeodactylum tricornutum, using different physiological, biochemical and molecular approaches. The results show that acclimation to QDs reduced the growth inhibition induced by nanoparticles in P. tricornutum cultures. The increase of glutathione observed at the end of the lag phase pointed to cellular stress. Transcriptional expression of selected stress responsive genes showed up-regulation in the QD-exposed algae. A comparison of the proteomes of exposed and unexposed cells highlighted a large number of differentially expressed proteins. To our knowledge, this is the first report on proteome analysis of a marine microalga exposed to nanoparticles.

Marine environmental research 111 , pp. 149–157

Keywords

Ecotoxicology, Phytoplankton Growth, Phaeodactylum tricornutum, Glutathione, Quantum dots, Nanoparticles, Proteomics

CNR authors

Basso Pozzi Barbara, Gabellieri Edi, Cioni Patrizia, Morelli Elisabetta

CNR institutes

IBF – Istituto di biofisica

ID: 334373

Year: 2015

Type: Articolo in rivista

Creation: 2015-10-01 12:25:43.000

Last update: 2021-04-12 10:15:28.000

External links

OAI-PMH: Dublin Core

OAI-PMH: Mods

OAI-PMH: RDF

DOI: 10.1016/j.marenvres.2015.06.018

External IDs

CNR OAI-PMH: oai:it.cnr:prodotti:334373

DOI: 10.1016/j.marenvres.2015.06.018

PubMed: 26183536

Scopus: 2-s2.0-849451156

ISI Web of Science (WOS): 000364726000017