CNR ExploRA

Articolo in rivista, 2016, ENG, 10.1186/s13068-016-0569-z

High-level expression of thermostable cellulolytic enzymes in tobacco transplastomic plants and their use in hydrolysis of an industrially pretreated Arundo donax L. biomass

Castiglia, Daniela; Sannino, Lorenza; Marcolongo, Loredana; Ionata, Elena; Tamburino, Rachele; De Stradis, Angelo; Cobucci-Ponzano, Beatrice; Moracci, Marco; La Cara, Francesco; Scotti, Nunzia

1 CNR-IBBR UOS Portici, National Research Council of Italy, Institute of Bio-sciences and BioResources, Research Division Portici, Via Università 133, 80055 Portici, NA, Italy. 2 CNR-IBBR UOS Naples, National Research Council of Italy, Institute of Biosciences and BioResources, Research Division Naples, Via P. Castellino 111, Naples, Italy. 3 CNR-IBAF UOS Napoli, National Research Council of Italy, Institute of Agro-environmental and Forest Biology, Research Division Naples, Via P. Castellino 111, Naples, Italy. 4 CNR-IPSP UOS Bari, National Research Council of Italy, Institute for Sustainable Plant Protection, Research Division Bari, Via Amendola 165/A, 70126 Bari, Italy.

Biofuels production from plant biomasses is a complex multi-step process with important economic burdens. Several biotechnological approaches have been pursued to reduce biofuels production costs. The aim of the present study was to explore the production in tobacco plastome of three genes encoding (hemi)cellulolytic enzymes from thermophilic and hyperthermophilic bacterium and Archaea, respectively, and test their application in the bioconversion of an important industrially pretreated biomass feedstock (A. donax) for production of second-generation biofuels.

Biotechnology for biofuels 9