Articolo in rivista, 2016, ENG, 10.1002/fsn3.383

Euglena gracilis paramylon activates human lymphocytes by upregulating pro-inflammatory factors

Rossella Russo, Laura Barsanti, Valter Evangelista, Anna M. Frassanito, Vincenzo Longo, Laura Pucci, Giuseppe Penno, Paolo Gualtieri

CNR- Istituto di Biologia e Biotecnologia Agraria: RR, VL, LP; CNR-IBF: LB, VE, AMF, PG; Dipartimento di Medicina Clinica e Sperimentale, Sezione Malattie Metaboliche, Università di Pisa: GP

The aim of this study was to verify the activation details and products of human lymphomonocytes, stimulated by different -glucans, that is Euglena paramylon,MacroGard®, and lipopolysaccharide. We investigated the gene expression of inflammation-related cytokines and mediators, transactivation of relevant transcription factors, and phagocytosis role in cell-glucan interactions, by means of RT-PCR, immunocytochemistry, and colorimetric assay. Our results show that sonicated and alkalized paramylon upregulates pro-inflammatoryfactors (NO, TNF-?,IL-6,and COX-2) in lymphomonocytes. A clear demonstration of this upregulation is the increased transactivation of NF-kB visualized by immunofluorescence microscopy. Phagocytosis assay showed that internalization is not a mandatory step for signaling cascade to be triggered, since immune activity is not present in the lymphomonocytes that have internalized paramylon granules and particulate MacroGard®. Moreover, the response of Euglena ?-glucan-activated lymphomonocytes is much greater than that induced by commercially used ?-glucans such as MacroGard®. Our in vitro results indicate that linear fibrous Euglena ?-glucan, obtained by sonication and alkaline treatment can act as safe and effective coadjutant of the innate immune system response.

Food Science & Nutrition , pp. 1–10

Keywords

Euglena, glucans, innate immunity, MacroGard®, paramylon

CNR authors

Frassanito Anna Maria, Russo Rossella, Evangelista Valtere, Gualtieri Paolo, Longo Vincenzo, Pucci Laura, Barsanti Laura

CNR institutes

IBBA – Istituto di biologia e biotecnologia agraria

ID: 361917

Year: 2016

Type: Articolo in rivista

Creation: 2016-12-05 17:26:24.000

Last update: 2022-11-29 13:05:14.000

External links

OAI-PMH: Dublin Core

OAI-PMH: Mods

OAI-PMH: RDF

DOI: 10.1002/fsn3.383

External IDs

CNR OAI-PMH: oai:it.cnr:prodotti:361917

DOI: 10.1002/fsn3.383