Articolo in rivista, 2016, ENG, 10.12681/mms.1693

Oocyte batch development and enumeration in the European anchovy (Engraulis encrasicolus)

Ferreri R.; Ganias K.; Genovese S.; Fontana I.; Giacalone G.; Bonanno A.; Mazzola S.; Aronica S.; Mangano S.; Basilone G.

Institute for Coastal and Marine Environment - National Research Council, (IAMC-CNR) Capo Granitola, Via del Mare, 3, Torretta Granitola, Campobello di Mazara (TP), 91021, Italy; School of Biology, Aristotle University of Thessaloniki, Thessaloniki, 54 124, Greece

An alternative method to the traditional hydrated oocyte (HO) method has been evaluated for the Sicilian anchovy, Engraulis encrasicolus. The method is based on the processing of ovarian whole mount images and the identification of the spawning batch in oocyte size frequency distributions and shows the advantage that it can be applied to various oocyte stages rather than strictly to the HO stage. Despite the peculiar elliptical shape of anchovy oocytes, this image analysis technique was fully successful since the yolked stage appeared to perform equally to the HO stage for anchovy batch fecundity measurements.

Mediterranean Marine Science 17 , pp. 670–677

Keywords

Anchovy, ovarian whole mounts, image analysis, elliptical egg shape, batch fecundity.

CNR authors

Bonanno Angelo, Basilone Gualtiero, Aronica Salvatore, Ferreri Rosalia, Genovese Simona, Giacalone Giovanni, Fontana Ignazio, Mangano Salvatore, Mazzola Salvatore

CNR institutes