CNR ExploRA

Articolo in rivista, 2018, ENG, 10.1016/j.phytochem.2018.06.015

Biochemical and biophysical characterization of water-soluble pectin from Opuntia ficus indica and its potential biological activity

Lefsih K, Giacomazza D, Passantino R, Costa MA, Bulone D, Mangione MR, Guarrasi V, Mingoia F, San Biagio PL, Madani K

Laboratoire de Biomathématiques, Biophysique, Biochimie et Scientométrie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000, Bejaia, Algeria; Département de Biochimie et Microbiologie, Faculté des Sciences Biologiques et des Sciences Agronomiques, Université de Tizi ouzou, 15000, Tizi ouzou, Algeria. Electronic address: klefsih@yahoo.fr; Istituto di Biofisica, Consiglio Nazionale delle Ricerche, Palermo, Italy; Istituto per lo Studio dei Materiali Nanostrutturati, Consiglio Nazionale delle Ricerche, Palermo, Italy; Laboratoire de Biomathématiques, Biophysique, Biochimie et Scientométrie, Faculté des Sciences de la Nature et de la Vie, Université de Bejaia, 06000, Bejaia, Algeria.

This work aims to fill the gap in the present knowledge about the structure of pectin from Opuntia ficus-indica. The water-soluble pectin (WSP) fraction, extracted with the Microwave Assisted Extraction (MAE), was further deproteinated (dWSP) and analyzed through several biophysical and biochemical techniques. HPSEC, light scattering and FTIR data showed that dWSP is low methylated high molecular weight pectin. The biochemical structure of dWSP, after methanolysis, silylation, carboxyl reduction showed that dWSP belongs to rhamnogalacturonan I class. Then, dWSP was heat-modified (HM) to obtain small-molecular weight deproteinated fraction (HM-dWSP). Both species, dWSP and HM-dWSP, were tested in LAN5 and NIH 3T3 model cells to study their biological effect. Results indicated that both dWSP and HM-dWSP exerted cytotoxic activity affecting selectively LAN5 cancer cells, without any effect on NIH 3T3 normal cells.

Phytochemistry 154 , pp. 47–55