Articolo in rivista, 2018, ENG, 10.1038/s41598-018-19558-w

Triggering molecular assembly at the mesoscale for advanced Raman detection of proteins in liquid

Banchelli, Martina; de Angelis, Marella; D'Andrea, Cristiano; Pini, Roberto; Matteini, Paolo

Natl Res Council IFAC CNR

An advanced optofluidic system for protein detection based on Raman signal amplification via dewetting and molecular gathering within temporary mesoscale assemblies is presented. The evaporation of a microliter volume of protein solution deposited in a circular microwell precisely follows an outward-receding geometry. Herein the combination of liquid withdrawal with intermolecular interactions induces the formation of self-assembled molecular domains at the solid-liquid interface. Through proper control of the evaporation rate, amplitude of the assemblies and time for spectral collection at the liquid edge are extensively raised, resulting in a local enhancement and refinement of the Raman response, respectively. Further signal amplification is obtained by taking advantage of the intense local electromagnetic fields generated upon adding a plasmonic coating to the microwell. Major advantages of this optofluidic method lie in the obtainment of high-quality, high-sensitivity Raman spectra with detection limit down to sub-micromolar values. Peculiarly, the assembled proteins in the liquid edge region maintain their native-like state without displaying spectral changes usually occurring when dried drop deposits are considered.

Scientific reports (Nature Publishing Group) 8

Keywords

self assembly, Raman spectroscopy, protein, microfluidic

CNR authors

De Angelis Marella, Matteini Paolo, Pini Roberto, Banchelli Martina, D Andrea Cristiano

CNR institutes

IFAC – Istituto di fisica applicata "Nello Carrara"