Articolo in rivista, 2019, ENG, 10.1515/pac-2018-1202

The effect of pore morphology on the catalytic performance of ?-glucosidase immobilized into mesoporous silica

Califano, Valeria; Costantini, Aniello; Silvestri, Brigida; Venezia, Virginia; Cimino, Stefano; Sannino, Filomena

Aniello Costantin, Brigida Silvestri, Virginia Venezia:Università degli Studi di Napoli Federico II, Napoli Filomena Sannino: Università degli studi di Napoli, Portici

?-Glucosidase (BG) was immobilized by adsorption on wrinkled silica nanoparticles (WSNs) and on tannic acid-templated mesoporous silica nanoparticles (TA-MSNPs). The effect induced by a different morphology of the pores of the sorbent on the catalytic performance of ?-glucosidase was investigated. A complete textural and morphological characterization of the two samples was performed by Brunauer-Emmett-Teller (BET) method, Fourier Transform Infrared (FT-IR) and transmission electron microscopy (TEM). The results demonstrated that the catalytic performance of the immobilized enzyme depends on the pores size of sorbent but a key factor is the pores morphology. In fact, the BG immobilized on WSNs and TA-MSNPs (BG/WSNs and BG/TA-MSNPs) shows in both cases good catalytic performances in cellobiose hydrolysis, but the catalyst with the best performance is BG/WSNs, in which the support exhibits a central-radial pore structure and a hierarchical trimodal micro-mesoporous pore size. This peculiar morphology allows the enzyme to settle in a place where the interactions with the walls are maximized, increasing its conformational rigidity. Furthermore, the enzyme is prevalently collocated in the interior of pore so that the pores are not completely capped.

Pure and applied chemistry (Print) 91 (10), pp. 1583–1592

Keywords

biomass conversion, enzyme immobilization, Eurasia 2018, kinetics, thermal stability, ?-glucosidase

CNR authors

Cimino Stefano, Califano Valeria

CNR institutes

IM – Istituto motori, IRC – Istituto di ricerche sulla combustione