Articolo in rivista, 2019, ENG, 10.3389/fpls.2019.01575

Distinct metabolic signals underlie clone by environment interplay in 'Nebbiolo' grapes over ripening

Pagliarani C., Boccacci P., Chitarra W., Cosentino E., Sandri M., Perrone I., Mori A., Cuozzo D., Nerva L., Rossato M., Zuccolotto P., Pezzotti M., Delledonne M., Mannini F., Gribaudo I., Gambino G.

Cosentino E., Mori A., Rossato M., Pezzotti M., Delledonne M : Department of Biotechnology, University of Verona, Verona, Italy; Sandri M.: DMS StatLab, University of Brescia, Brescia, Italy; Zuccolotto P.: Big&Open Data Innovation Laboratory, University of Brescia, Brescia, Italy; Cuozzo D.: Department of Agricultural, Forest and Food Sciences, University of Torino, Grugliasco, Italy

"Several research studies were focused to understand how grapevine cultivars respond to environment; nevertheless, the biological mechanisms tuning this phenomenon need to be further deepened. Particularly, the molecular processes underlying the interplay between clones of the same cultivar and environment were poorly investigated. To address this issue, we analyzed the transcriptome of berries from three ""Nebbiolo"" clones grown in different vineyards, during two ripening seasons. RNA-sequencing data were implemented with analyses of candidate genes, secondary metabolites, and agronomical parameters. This multidisciplinary approach helped to dissect the complexity of clone × environment interactions, by identifying the molecular responses controlled by genotype, vineyard, phenological phase, or a combination of these factors. Transcripts associated to sugar signalling, anthocyanin biosynthesis, and transport were differently modulated among clones, according to changes in berry agronomical features. Conversely, genes involved in defense response, such as stilbene synthase genes, were significantly affected by vineyard, consistently with stilbenoid accumulation. Thus, besides at the cultivar level, clone-specific molecular responses also contribute to shape the agronomic features of grapes in different environments. This reveals a further level of complexity in the regulation of genotype × environment interactions that has to be considered for orienting viticultural practices aimed at enhancing the quality of grape productions."

Frontiers of plant science 10

Keywords

candidate gene expression, clones, secondary metabolism, sugar signalling, Vitis vinifera

CNR authors

Nerva Luca, Chitarra Walter, Mannini Franco, Gribaudo Ivana, Gambino Giorgio, Boccacci Paolo, Perrone Irene, Pagliarani Chiara

CNR institutes

IPSP – Istituto per la Protezione Sostenibile delle Piante