Articolo in rivista, 2020, ENG, 10.1371/journal.pone.0237111

aTBP: A versatile tool for fish genotyping

Giani S.; Silletti S.; Gavazzi F.; Morello L.; Spinsanti G.; Parati K.; Breviario D.

Department Scienze Bioagroalimentari, Istituto Biologia e Biotecnologia Agraria, National Research Council, Milano, , , Italy; Department Scienze Bioagroalimentari, Istituto Biologia e Biotecnologia Agraria, National Research Council, Milano, , , Italy; Department of Life Sciences, University of Siena, Siena, , Italy; Istituto Sperimentale Italiano Lazzaro Spallanzani, Rivolta d'Adda (CR), , Italy

Animal Tubulin-Based-Polymorphism (aTBP), an intron length polymorphism method recently developed for vertebrate genotyping, has been successfully applied to the identification of several fish species. Here, we report data that demonstrate the ability of the aTBP method to assign a specific profile to fish species, each characterized by the presence of commonly shared amplicons together with additional intraspecific polymorphisms. Within each aTBP profile, some fragments are also recognized that can be attributed to taxonomic ranks higher than species, e.g. genus and family. Versatility of application across different taxonomic ranks combined with the presence of a significant number of DNA polymorphisms, makes the aTBP method an additional and useful tool for fish genotyping, suitable for different purposes such as species authentication, parental recognition and detection of allele variations in response to environmental changes.

PloS one 15 , pp. e0237111–?

Keywords

TBP, molecular markers, fish, genome profiling, vertebrate taxonomy

CNR authors

Giani Silvia, Gavazzi Floriana, Morello Laura Emma Maria, Breviario Diego

CNR institutes

IBBA – Istituto di biologia e biotecnologia agraria