Articolo in rivista, 2021, ENG, 10.1007/s00421-020-04527-x
Mrakic-Sposta S.; Gussoni M.; Dellanoce C.; Marzorati M.; Montorsi M.; Rasica L.; Pratali L.; D'Angelo G.; Martinelli M.; Bastiani L.; Di Natale L.; Vezzoli A.
CNR-IFC, Milano, Italy ; SCITEC-CNR, Milano, Italy; CNR-IBFM, Milano, Italy; CNR-IBMT, Milano, Italy; University of Calgary, Calgary, Canada; CNR-IFC, Pisa, Italy; CNR-IFC, Pisa, Italy; CNR-ISTI, Pisa, Italy; CNR-IFC, Pisa, Italy; Centre Hospitalier Universitarie de Dijon-Bourgogne, Dijon, France; CNR-IFC, Milano, Italy
Purpose High altitude results in lower barometric pressure and hence partial pressure of O2 decrease can lead to several molecular and cellular changes, such as generation of reactive oxygen species (ROS). Electron Paramagnetic Resonance technique was adopted in the field, to evaluate the effects of acute and sub-acute hypobaric hypoxia (HH) on ROS production by micro-invasive method. Biological biomarkers, indicators of oxidative stress, renal function and inflammation were investigated too. Methods Fourteen lowlander subjects (mean age 27.3 ± 5.9 years) were exposed to HH at 3269 m s.l. ROS production, related oxidative damage to cellular components, systemic inflammatory response and renal function were determined through blood and urine profile performed at 1st, 2nd, 4th, 7th, and 14th days during sojourn. Results Kinetics of changes during HH exposition showed out significant (range p < 0.05-0.0001) increases that at max corresponds to 38% for ROS production rate, 140% for protein carbonyl, 44% for lipid peroxidation, 42% for DNA damage, 200% for inflammatory cytokines and modifications in renal function (assessed by neopterin concentration: 48%). Conversely, antioxidant capacity significantly (p < 0.0001) decreased - 17% at max. Conclusion This 14 days in-field study describes changes of oxidative-stress biomarkers during HH exposure in lowlanders. The results show an overproduction of ROS and consequent oxidative damage to protein, lipids and DNA with a decrease in antioxidant capacity and the involvement of inflammatory status and a transient renal dysfunction. Exposure at high altitude induces a hypoxic condition during acute and sub-acute phases accompanied by molecular adaptation mechanism indicating acclimatization.
European journal of applied physiology (Print) 121 , pp. 297–306
Acute mountain sickness, Electron paramagnetic resonance, Hypobaric hypoxia, High-performance liquid chromatography, oxidative stress
Gussoni Maristella, D Angelo Gennaro, Rasica Letizia, Martinelli Massimo, Pratali Lorenza, Dellanoce Cinzia Carla, Vezzoli Alessandra, Marzorati Mauro, Bastiani Luca, Mrakic Sposta Simona
IBFM – Istituto di bioimmagini e fisiologia molecolare, IFC – Istituto di fisiologia clinica, ISTI – Istituto di scienza e tecnologie dell'informazione "Alessandro Faedo", SCITEC – Istituto di Scienze e Tecnologie Chimiche "Giulio Natta"
ID: 434151
Year: 2021
Type: Articolo in rivista
Creation: 2020-10-15 15:57:52.000
Last update: 2022-01-25 13:28:30.000
External links
OAI-PMH: Dublin Core
OAI-PMH: Mods
OAI-PMH: RDF
DOI: 10.1007/s00421-020-04527-x
URL: https://link.springer.com/article/10.1007/s00421-020-04527-x
External IDs
CNR OAI-PMH: oai:it.cnr:prodotti:434151
DOI: 10.1007/s00421-020-04527-x
ISI Web of Science (WOS): 000577949500001
Scopus: 2-s2.0-85092566809