Articolo in rivista, 2021, ENG, 10.3390/ijms22020820
Della Camera, Giacomo; Lipsa, Dorelia; Mehn, Dora; Italiani, Paola; Boraschi, Diana; Gioria, Sabrina
European Commiss; Natl Res Council CNR
This study aims to provide guidelines to design and perform a robust and reliable physical-chemical characterization of liposome-based nanomaterials, and to support method development with a specific focus on their inflammation-inducing potential. Out of eight differently functionalized liposomes selected as "case-studies", three passed the physical-chemical characterization (in terms of size-distribution, homogeneity and stability) and the screening for bacterial contamination (sterility and apyrogenicity). Although all three were non-cytotoxic when tested in vitro, they showed a different capacity to activate human blood cells. HSPC/CHOL-coated liposomes elicited the production of several inflammation-related cytokines, while DPPC/CHOL- or DSPC/CHOL-functionalized liposomes did not. This work underlines the need for accurate characterization at multiple levels and the use of reliable in vitro methods, in order to obtain a realistic assessment of liposome-induced human inflammatory response, as a fundamental requirement of nanosafety regulations.
International journal of molecular sciences (Online) 22 (2)
nanomaterial, liposome, safety assessment, nanomedicine, particle size distribution, physicochemical characterization, Limulus Amoebocyte Lisate (LAL), endotoxin, inflammation, interleukin, cytokines
Boraschi Diana, Italiani Paola
ID: 449527
Year: 2021
Type: Articolo in rivista
Creation: 2021-03-24 15:20:08.000
Last update: 2021-03-24 19:35:20.000
CNR authors
CNR institutes
External IDs
CNR OAI-PMH: oai:it.cnr:prodotti:449527
DOI: 10.3390/ijms22020820
ISI Web of Science (WOS): 000611301800001