Articolo in rivista, 2021, ENG, 10.3390/ijms22020820

A Step-by-Step Approach to Improve Clinical Translation of Liposome-Based Nanomaterials, a Focus on Innate Immune and Inflammatory Responses

Della Camera, Giacomo; Lipsa, Dorelia; Mehn, Dora; Italiani, Paola; Boraschi, Diana; Gioria, Sabrina

European Commiss; Natl Res Council CNR

This study aims to provide guidelines to design and perform a robust and reliable physical-chemical characterization of liposome-based nanomaterials, and to support method development with a specific focus on their inflammation-inducing potential. Out of eight differently functionalized liposomes selected as "case-studies", three passed the physical-chemical characterization (in terms of size-distribution, homogeneity and stability) and the screening for bacterial contamination (sterility and apyrogenicity). Although all three were non-cytotoxic when tested in vitro, they showed a different capacity to activate human blood cells. HSPC/CHOL-coated liposomes elicited the production of several inflammation-related cytokines, while DPPC/CHOL- or DSPC/CHOL-functionalized liposomes did not. This work underlines the need for accurate characterization at multiple levels and the use of reliable in vitro methods, in order to obtain a realistic assessment of liposome-induced human inflammatory response, as a fundamental requirement of nanosafety regulations.

International journal of molecular sciences (Online) 22 (2)

Keywords

nanomaterial, liposome, safety assessment, nanomedicine, particle size distribution, physicochemical characterization, Limulus Amoebocyte Lisate (LAL), endotoxin, inflammation, interleukin, cytokines

CNR authors

Boraschi Diana, Italiani Paola

CNR institutes

IBBC – Istituto di Biochimica e Biologia Cellulare

ID: 449527

Year: 2021

Type: Articolo in rivista

Creation: 2021-03-24 15:20:08.000

Last update: 2021-03-24 19:35:20.000

External links

OAI-PMH: Dublin Core

OAI-PMH: Mods

OAI-PMH: RDF

DOI: 10.3390/ijms22020820

External IDs

CNR OAI-PMH: oai:it.cnr:prodotti:449527

DOI: 10.3390/ijms22020820

ISI Web of Science (WOS): 000611301800001