Articolo in rivista, 2021, ENG, 10.1016/j.foodres.2021.110386

Triticum monococcum amylase trypsin inhibitors possess a reduced potential to elicit innate immune response in celiac patients compared to Triticum aestivum

Iacomino G.; Rotondi Aufiero V.; Di Stasio L.; Picascia S.; Iannaccone N.; Giardullo N.; Troncone R.; Gianfrani C.; Mamone G.; Mazzarella G.

Institute of Food Sciences, CNR, Avellino, Institute of Food Sciences, CNR, Avellino, Italy, , Italy; Institute of Biochemistry and Cell Biology, CNR, Napoli, Institute of Biochemistry and Cell Biology, CNR, Napoli, Italy, , Italy; E.L.F.I.D, University "Federico II" Napoli, E.L.F.I.D, University "Federico II" Napoli, Italy, , Italy; Gastroenterology Department, S. G. Moscati Hospital, Avellino, Gastroenterology Department, S. G. Moscati Hospital, Avellino, Italy, , Italy; Department of Translational Medical Science and E.L.F.I.D, University "Federico II" Napoli, Department of Translational Medical Science and E.L.F.I.D, University "Federico II" Napoli, Italy, , Italy

Scope: Several studies reported a role of amylase/trypsin-inhibitors (ATIs) of common wheat species in promoting immune reactions. Here, we investigated in celiac disease (CD), the immunogenic properties of ATIs from diploid compared to common hexaploid wheats after an in vitro proteolytic hydrolysis. Methods and Results: ATIs purified from two lines of diploid Triticum monococcum (TM), Monlis and Norberto-ID331, and from Triticum aestivum (TA), Sagittario, were digested with pepsin-chymotrypsin (PC) enzymes and analyzed using a proteomic approach, and subsequently their immune stimulatory properties were investigated on jejunal biopsies and T-cell lines from CD patients. No significant expression of IL-8 and TNF-? were detected on biopsies cultured with ATIs from TM in comparison with ATIs from TA. No significant IFN-? production was observed in intestinal gliadin- raised T-cells in response to ATIs from both TM and TA wheats. Proteomic results revealed that both TM ATIs showed reduced stability to proteolytic enzymes compared to TA ones. Conclusion: TM ATIs are substantially different from those of TA, showing a reduced ability to trigger the innate immunity in CD and a higher susceptibility to enzymatic hydrolysis.

Food research international 145 , pp. 110386–?

Keywords

ATIs, Triticum monococcum, amylase trypsin inhibitors, innate immune response, celiacs

CNR authors

Rotondi Aufiero Vera, Di Stasio Luigia, Picascia Stefania, Iacomino Giuseppe, Gianfrani Carmela, Mazzarella Giuseppe, Mamone Gianfranco

CNR institutes

ISA – Istituto di Scienze dell'Alimentazione