Articolo in rivista, 2021, ENG, 10.1016/j.ejpb.2021.07.015

Dopamine-loaded lipid based nanocarriers for intranasal administration of the neurotransmitter: a comparative study

Adriana Trapani; Elvira De Giglio; Stefania Cometa; Maria Addolorata Bonifacio; Laura Dazzi; Sante Di Gioia; Md Niamat Hossain; Rosalia Pellitteri; Sophia G Antimisiaris ; Massimo Conese

Department of Pharmacy-Drug Sciences, University of Bari "Aldo Moro", Bari, Italy. Electronic address: adriana.trapani@uniba.it. Chemistry Department, University of Bari "Aldo Moro", via Orabona, 4, Bari 70125, Italy. Jaber Innovation s.r.l., Rome 00144, Italy. Department of Life and Environmental Sciences, Section of Neuroscience and Anthropology, University of Cagliari, Monserrato (Cagliari), Italy. Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy. Electronic address: sante.digioia@unifg.it. Department of Medical and Surgical Sciences, University of Foggia, Foggia, Italy. Institute for Biomedical Research and Innovation (IRIB-CNR), Catania 95126, Italy. Laboratory of Pharm. Technology, Dept. of Pharmacy, School of Health Sciences, University of Patras, Rio 26504, Greece; Foundation for Research and Technology Hellas, Institute of Chemical Engineering Sciences, FORTH/ICE-HT, Rio 26504, Greece.

Both dopamine (DA) loaded Solid Lipid Nanoparticles (SLN) and liposomes (Lip), designed for intranasal administration of the neurotransmitter as an innovative Parkinson disease treatment, were already characterized in vitro in some extent by us (Trapani et al., 2018a and Cometa et al., 2020, respectively). Herein, to gain insight into the structure of SLN, X-ray Photoelectron Spectroscopy Analysis was carried out and DA-SLN (SLN 1) were found to exhibit high amounts of the neurotransmitter on the surface, whereas the external side of Glycol Chitosan (GCS) containing SLN (SLN 2) possessed only few amounts. However, SLN 2 were characterized by the highest encapsulation DA efficiency (i.e., 81%). Furthermore, in view of intranasal administration, mucoadhesion tests in vitro were also conducted for SLN and Lip formulations, evidencing high muchoadesive effect exerted by SLN 2. Concerning ex-vivo studies, SLN and Lip were found to be safe for Olfactory Ensheathing Cells and fluorescent SLN 2 were taken up in a dose-dependent manner reaching the 100% of positive cells, while Lip 2 (chitosan-glutathione-coated) were internalised by 70% OECs with six-times more lipid concentration. Hence, SLN 2 formulation containing DA and GCS may constitute interesting formulations for further studies and promising dosage form for non-invasive nose-to-brain neurotransmitter delivery

European journal of pharmaceutics and biopharmaceutics 167 , pp. 189–200

Keywords

Cytotoxicity; Dopamine; Liposomes; Olfactory Ensheathing cells; Solid lipid nanoparticles; Uptake; X-Ray Photoelectron Spectroscopy Analysis

CNR authors

Pellitteri Rosalia Maria Cristina

CNR institutes

IRIB – Istituto per la Ricerca e l'Innovazione Biomedica