Articolo in rivista, 2004, ENG

Liquid chromatographic analysis of Alternaria toxins in carrots

Solfrizzo M., De Girolamo A., Vitti C., Visconti A., van den Bulk R.

CNR, Institute of Sciences of Food Production; Plant Research International, Wageningen, The Netherlands

A liquid chromatographic method for the analysis of Alternaria radicina and A. alternata toxins in carrots was developed. Toxins were extracted from carrot with an acidified mixture of water+methanol+acetonitrile. The filtered extract was divided in two parts that were purified by solid phase extraction on a C18 column for the analysis of radicinin (RAD), altertoxin-I (ATX-I), alternariol (AOH), and alternariol methyl ether (AME) and on a polymeric OasisÒ HLB column for tenuazonic acid (TeA), respectively. Toxins were quantified by reversed phase liquid chromatography with ultraviolet diode array detector by using two consecutive isocratic mixtures of acetonitrile-sodium dihydrogen phosphate solution. Mean recoveries of TeA, ATX-I, AME, RAD, and AOH from carrots spiked at levels between 0.5 and 3.0 µg/g were 69, 71, 90, 36, and 78%, with mean within laboratory repeatability of 14, 5, 4, 6, and 18%, respectively. The mean between laboratory reproducibility for the determination of TeA, ATX-I, AME, and RAD in spiked samples were 25, 22, 6, and 12%, respectively. Limits of detection (signal-to-noise ratio of 3) for RAD, TeA, ATX-I, AME, and AOH were 0.006, 0.02, 0.02, 0.01, and 0.005 µg/g, respectively. Radicinin was detected (0.16 – 13.9 µg/g) in 3 out of 266 carrot samples produced under organic conditions in three European locations, whereas A. alternata mycotoxins were not found in all tested samples.

Journal of AOAC International 87 , pp. 101–106

Keywords

Alternaria, carote, micotossine, metodo analitico

CNR authors

Vitti Carolina, De Girolamo Annalisa, Solfrizzo Michele, Visconti Angelo

CNR institutes

ISPA – Istituto di scienze delle produzioni alimentari

ID: 46253

Year: 2004

Type: Articolo in rivista

Creation: 2009-06-16 00:00:00.000

Last update: 2018-12-04 16:42:19.000

External links

OAI-PMH: Dublin Core

OAI-PMH: Mods

OAI-PMH: RDF

External IDs

CNR OAI-PMH: oai:it.cnr:prodotti:46253