Articolo in rivista, 2022, ENG, 10.3390/photonics9090627
Senesi G.S.; De Pascale O.; Spolon Marangoni B.; Rodrigues Lima Caires A.; Nicoldelli G.; Pantaleo V.; Leonetti P.
CNR ISTP - Institute for Plasma Science and Technology, Research Unit of Bari, Italy; Physics Institute, Federal University of Mato Grosso do Sul, Campo Grande, MS, Brazil; Departamento de Física, Federal University of Santa Catarina, Florianopolis, SC, Brazil; CNR IPSP - Institute for Sustainable Plant Protection, Research Unit of Bari, Italy.
Recently, studies on climate change have highlighted the central role of photosynthetic mechanisms in the defense response of plants to abiotic and biotic stresses. Photo-sensing and photo-activation are innovative technologies applied for the early detection of plant pathogens in order to prevent the dramatic impact they may have on plants. Chlorophyll Fluorescence Imaging (CFI) and Laser-Induced Breakdown Spectroscopy (LIBS) analytical techniques can be used to evaluate the amount of chlorophyll in plants, which can be altered in the case of biotic and abiotic stresses. In this work, both techniques were applied to two pathogenic model systems, i.e., roots of susceptible tomato plants infected by Meloidogyne incognita and Nicotiana benthamiana plants infected by cymbidium ringspot virus. Experimental evidence is provided and discussed showing that specific application protocols of both methods can be used successfully for the early detection of symptoms of the pathogen attacks of Meloidogyne incognita on tomato roots and of cymbidium ringspot virus infected plants. In particular, a decrease in chlorophyll content was measured by fluorescence imaging, and an increase in Mg++ content was determined by LIBS in both the leaves and stems of infected tomato plants and the leaves of infected plants, with respect to control (non-infected) plants. Thus, the two techniques used have been shown to be able to discriminate satisfactorily between control and infected plants and to provide some insight on the underlying mechanisms of plant defenses again nematodes and viruses.
Photonics 9 , pp. 1–14
tomato, Meloidogyne incognita root knot nematode, tobacco, cymbidium ringspot virus, chlorophyll fluorescence imaging, laser-induced breakdown spectroscopy
De Pascale Olga, Pantaleo Vitantonio, Senesi Giorgio Saverio, Leonetti Paola
IPSP – Istituto per la Protezione Sostenibile delle Piante, ISTP – Istituto per la Scienza e Tecnologia dei Plasmi
ID: 470513
Year: 2022
Type: Articolo in rivista
Creation: 2022-09-06 12:33:23.000
Last update: 2022-10-11 19:49:38.000
External IDs
CNR OAI-PMH: oai:it.cnr:prodotti:470513
DOI: 10.3390/photonics9090627
Scopus: 2.0-85138656341
ISI Web of Science (WOS): 000858714800001