Articolo in rivista, 2021, ENG, 10.1016/j.saa.2021.119813
Bonsignore, Martina; Trusso, Sebastiano; De Pasquale, Claudia; Ferlazzo, Guido; Allegra, Alessandro; Innao, Vanessa; Musolino, Caterina; Franco, Domenico; De Plano, Laura Maria; Guglielmino, Salvatore Pietro Paolo; Neri, Fortunato; Fazio, Enza
Univ Messina; CNR; Univ Messina; Univ Messina; Univ Messina
Trusted methods for identifying different Multiple Myeloma (MM) cells and their biological diversity due to their immunophenotypic variety are often little detailed and difficult to find in literature. In this work, we show that micro-Raman spectroscopy can be used to highlight if there is a certain degree of distinction or correlation between the MM subtype plasmacells in relation to the cluster of differentiation (CD45 +/CD38+/CD138-) and (CD45-/CD38+/CD138+). After taking samples from the bone marrow of patients with Multiple Myeloma, the PCs were sorted by flow cytometry, selecting the most common CD of the disease, i.e. CD 45, CD38 and CD138. Some spectral differences are observed comparing the Raman spectra of the two set of samples investigated. To better define in which spectral regions there are greater differences and, therefore, to which biological contributions the changes refers, we also explored the principal component analysis (PCA) of the collected Raman data. The spectral variations between the different sorted cells have been highlighted by plotting loading vectors PC1 and PC2, which shows a net differentiation between the two set of cells. Ultimately, the differences shown by PCA have been associated with the spectral variations observed and explained in terms of changes of proteins and lipid contributions. Thus, the differentiation of Multiple Myeloma subtype plasma cells by confocal micro-Raman spectroscopy can be proposed as a diagnostic tool in the speeding up of cell identification, assessing the intracellular biochemical changes that take place in cancer cells. (c) 2021 Published by Elsevier B.V.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy (Print) 258
Raman spectroscopy, PCA, Multiple Myeloma, Cell sorting
ID: 485675
Year: 2021
Type: Articolo in rivista
Creation: 2023-08-23 12:29:33.000
Last update: 2023-08-23 12:29:33.000
CNR authors
CNR institutes
External IDs
CNR OAI-PMH: oai:it.cnr:prodotti:485675
DOI: 10.1016/j.saa.2021.119813
ISI Web of Science (WOS): 000663171800002