CNR ExploRA

Articolo in rivista, 2023, ENG

Meta-analysis of transcriptome reveals key genes relating to oil quality in olive

AliAkbar Asadi, Vahid Shariati, Soraya Mousavi, Roberto Mariotti, Mehdi Hosseini Mazinani

National Institute of Genetic Engineering and Biotechnology (NIGEB), Shahrak-e Pajoohesh, Km 15, Tehran - Karaj Highway, PO Box 14965161, Tehran, Iran. Institute of Biosciences and Bioresources, National Research Council, 06128 Perugia, Italy.

Background Olive oil contains monounsaturated oleic acid up to 83% and phenolic compounds, making it an excellent source of fat. Due to its economic importance, the quantity and quality of olive oil should be improved in parallel with international standards. In this study, we analyzed the raw RNA-seq data with a meta-analysis approach to identify important genes and their metabolic pathways involved in olive oil quality. Results A deep search of RNA-seq published data shed light on thirty-nine experiments associated with the olive transcriptome, four of these proved to be ideal for meta-analysis. Meta-analysis confrmed the genes identifed in previous studies and released new genes, which were not identifed before. According to the IDR index, the meta-analysis had good power to identify new diferentially expressed genes. The key genes were investigated in the metabolic pathways and were grouped into four classes based on the biosynthetic cycle of fatty acids and factors that afect oil quality. Galactose metabolism, glycolysis pathway, pyruvate metabolism, fatty acid biosynthesis, glycerolipid metabolism, and terpenoid backbone biosynthesis were the main pathways in olive oil quality. In galactose metabolism, rafnose is a suitable source of carbon along with other available sources for carbon in fruit development. The results showed that the biosynthesis of acetyl-CoA in glycolysis and pyruvate metabolism is a stable pathway to begin the biosynthesis of fatty acids. Key genes in oleic acid production as an indicator of oil quality and critical genes that played an important role in production of triacylglycerols were identifed in diferent developmental stages. In the minor compound, the terpenoid backbone biosynthesis was investigated and important enzymes were identifed as an interconnected network that produces important precursors for the synthesis of a monoterpene, diterpene, triterpene, tetraterpene, and sesquiterpene biosynthesis. Conclusions The results of the current investigation can produce functional data related to the quality of olive oil and would be a useful step in reducing the time of cultivar screening by developing gene specifc markers in olive breeding programs, releasing also new genes that could be applied in the genome editing approach.

BMC genomics