RESULTS FROM 1 TO 20 OF 232

2023, Articolo in rivista, ENG

A combined analysis based on microbial communities and volatile organic compounds as a tool to study soil quality in an urban environment

Neri L., Sillo F., Zampieri E., Lumini E., Marturano G., Degli Esposti C., Petruzzelli G., Gioli B., Zaldei A., Baraldi R., Balestrini R.

Soil play crucial roles in providing relevant ecosystem services for a health planet. Particularly, soil microorganisms are relevant for the terrestrial ecosystem functioning. Anthropogenic activities, such as urbanisation, have an impact on the soil microorganism diversity that is considered an indicator of soil quality. In this scenario, urban soils are becoming a rising research topic and exploring associated microorganisms in urban greenspaces is an essential step toward a sustainable development. Importance of soil in the release and uptake of volatile organic compounds (VOCs) between ecosystems has been also established. Here, two approaches, based on DNA metabarcoding and VOC profiling, were used to characterize flowerbed urban soils located in an Italian city.

Pedosphere (Online)

2023, Contributo in volume, ENG

Modelling of Biogenic Volatile Organic Compounds Emissions Using a Detailed Vegetation Inventory Over a Southern Italy Region

Silibello C., Finardi S., Pepe N., Baraldi R., Ciccioli P., Mircea M., Ciccioli P.

Biogenic Volatile Organic Compounds (BVOC) play a key role in ozone-forming reactions and are precursors of secondary organic aerosols that absorb and scatter the atmospheric radiation, affect precipitations by acting as cloud condensation nuclei and increase PM concentrations affecting thus the human health. Therefore, it is important to develop models that can accurately estimate such emissions. BVOC emissions show wide variability depending on the distributions of plant species, their emission potentials and seasonal behavior. This work presents further developments of Plant-Specific Emission Model (PSEM) and its application over Campania region (Southern Italy) considering a detailed recognition of tree species, natural vegetation, and agricultural land performed at a very high spatial resolution (200 m). Its BVOC emissions were compared with those provided by Model of Emissions of Gases and Aerosol from Nature (MEGAN, v2.04) evidencing significant differences, particularly for isoprene. Such emissions were further used to feed a Chemical Transport Model (CTM) applied over the Vesuvius area, where vertical profiles of BVOC concentrations were measured by tethered balloon soundings. The comparison between the observed and predicted BVOC concentrations evidenced the capability of the developed model to estimate the biogenic emissions and highlighted the relevance of an accurate inventory of the vegetation.

DOI: 10.1007/978-3-031-12786-1_38

2023, Articolo in rivista, ENG

The potential impact of biogenic volatile organic compounds (BVOCs) from terrestrial vegetation on a Mediterranean area using two different emission models

Ciccioli P., Silibello C., Finardi S., Pepe N., Ciccioli P., Rapparini F., Neri L., Fares S., Brilli F., Mircea M., Magliulo E., Baraldi R.

Biogenic Volatile Organic Compounds (BVOCs) emitted from vegetation are precursors of ozone (O3), photochemical oxidants and secondary organic aerosols (SOA) in the lower troposphere. The interaction between urban polluted air plumes originated along the coasts of the Mediterranean basin with biogenic emission occurring inland contributes to the occurrence of exceedances of the air quality standards for O3 and fine suspended particles (PM10, PM2.5). This interaction is favoured by prevailing sea-land breeze circulation during persistent high-pressure conditions. The actual contribution of BVOC to photochemical pollution is still uncertain because the approaches used to assess the emissions from terrestrial vegetation are quite different. There is some evidence in literature that BVOC emissions from Mediterranean vegetation is not accurately estimated by models based on the plant functional types (PFT) approach. To investigate these issues, a Plant Specific Emission Model (PSEM) was developed and applied to the Campania region in Southern Italy, for which a detailed vegetation inventory has been built. BVOC emission maps estimated by PSEM were compared with those generated using a PFT methodology, evidencing significant differences. BVOC emissions from the two models were then used to predict the concentrations of precursors and products of photochemical smog pollution over the Gulf of Naples (Italy) using a chemical-transport model. Simulations were performed during a period characterised by high pressure conditions that favour an enhanced O3 production under a sea-breeze circulation regime. VOCs concentration profiles predicted by the two models were compared with field data collected over Mount Vesuvius using a tethered balloon coupled with a PTR-MS. The results proved the better capability of PSEM to predict the concentrations of many BVOCs, including isoprene and some of its primary oxidation products over the measuring site, and suggested the significant potential of BVOC emission to produce SOA over the gulf of Naples.

Agricultural and forest meteorology (Print) 328

DOI: 10.1016/j.agrformet.2022.109255

2022, Articolo in rivista, ENG

Root Exposure to 5-Aminolevulinic acid (ALA) Affects Leaf Element Accumulation, Isoprene Emission, Phytohormonal Balance, and Photosynthesis of Salt-Stressed Arundo donax

Brilli F.;Pignattelli S.;Baraldi R.; Neri L.; Pollastri S:; Gonnelli C.; Giovannelli A.; Loreto F.; Cocozza C.

Arundo donax has been recognized as a promising crop for biomass production on marginal lands due to its superior productivity and stress tolerance. However, salt stress negatively impacts A. donax growth and photosynthesis. In this study, we tested whether the tolerance of A. donax to salinity stress can be enhanced by the addition of 5-aminolevulinic acid (ALA), a known promoter of plant growth and abiotic stress tolerance. Our results indicated that root exposure to ALA increased the ALA levels in leaves along the A. donax plant profile. ALA enhanced Na+ accumulation in the roots of salt- stressed plants and, at the same time, lowered Na+ concentration in leaves, while a reduced callose amount was found in the roottissue. ALA also improved the photosynthetic performance of salt-stressed apical leaves by stimulating stomatal opening and preventing an increase in the ratio between abscisic acid (ABA) and indol-3-acetic acid (IAA), without affecting leaf methanol emission and plant growth. Supply of ALA to the roots reduced isoprene fluxes from leaves of non-stressed plants, while it sustained isoprene fluxes along the profile of salt-stressed A. donax. Thus, ALA likely interacted with the methylerythritol 4-phosphate (MEP) pathway and modulate the synthesis of either ABA or isoprene under stressful conditions. Overall, our study highlights the effectiveness of ALA supply through soil fertirrigation in preserving the young apical developing leaves from the detrimental effects of salt stress, thus helping of A. donax to cope with salinity and favoring the recovery of the whole plant once the stress is removed.

International journal of molecular sciences (Print) 23 (8), pp. Article number 4311

DOI: 10.3390/ijms23084311

2022, Articolo in rivista, ENG

Exposure to different light intensities affects emission of volatiles and accumulations of both pigments and phenolics in Azolla filiculoides

Brilli F., K.G. Srikanta Dani, Pasqualini S., Costarelli A., Cannavò S., Paolocci F., Chini Zittelli G., Mugnai G., Baraldi R., Loreto F.

Many agronomic trials demonstrated the nitrogen-fixing ability of the ferns Azolla spp. and its obligate cyanobiont Trichormus azollae. In this study, we have screened the emission of volatile organic compounds (VOCs) and analyzed pigments (chlorophylls, carotenoids) as well as phenolic compounds in Azolla filiculoides-T. azollae symbionts exposed to different light intensities. Our results revealed VOC emission mainly comprising isoprene and methanol (82% and 13% of the overall blend, respectively). In particular, by dissecting VOC emission from A. filiculoides and T. azollae, we found that the cyanobacterium does not emit isoprene, whereas it relevantly contributes to the methanol flux. Enhanced isoprene emission capacity (15.95 ± 2.95 nmol m2 s1), along with increased content of both phenolic compounds and carotenoids, was measured in A. filiculoides grown for long-term under high (700 ?mol m2 s1) rather than medium (400 ?mol m2 s1) and low (100 ?mol m2 s1) light intensity. Moreover, lightresponses of chlorophyll fluorescence demonstrated that A. filiculoides was able to acclimate to high growth light. However, exposure of A. filiculoides from low (100 ?mol m2 s1) to very high light (1000 ?mol m2 s1) did not affect, in the short term, photosynthesis, but slightly decreased isoprene emission and leaf pigment content whereas, at the same time, dramatically raised the accumulation of phenolic compounds (i.e. deoxyanthocyanidins and phlobaphenes). Our results highlight a coordinated photoprotection mechanism consisting of isoprene emission and phenolic compounds accumulation employed by A. filiculoides to cope with increasing light intensities. 1 | INTRODUCTION Species belonging

Physiologia plantarum (Online) 174 (1), pp. e13619

DOI: 10.1111/ppl.13619

2021, Articolo in rivista, ENG

Stress markers and physiochemical responses of the Mediterranean shrub Phillyrea angustifolia under current and future drought and ozone scenarios

Pellegrini E.; Cotrozzi L.; Neri L.; Baraldi R.; Carrari E.; Nali C.; Lorenzini G.; Paoletti E.; Hoshika Y.

Mediterranean plants are particularly threatened by the exacerbation of prolonged periods of summer drought and increasing concentrations of ground-level ozone (O3). The aims of the present study were to (i) test if selected markers (i.e., reactive oxygen species, ROS; malondialdehyde, MDA; photosynthetic pigments) are able to discriminate the oxidative pressure due to single and combined stress conditions, and (ii) elucidate the physiochemical adjustments adopted by Phillyrea angustifolia (evergreen woody species representative of the maquis, also known as narrow-leaved mock privet) to perceive and counter to drought and/or O3. Plants were grown from May to October under the combination of two levels of water irrigation [i.e., well-watered (WW) and waterstressed (WS)] and three levels of O3 [i.e., 1.0, 1.5 and 2.0 times the ambient air concentrations, i.e. AA (current O3 scenario), 1.5 × AA and 2.0 × AA (future O3 scenarios), respectively], using a new-generation O3 Free Air Controlled Exposure (FACE) system. Overall, this species appeared relatively sensitive to drought (e.g., net CO2 assimilation rate and stomatal conductance significantly decreased, as well as total chlorophyll and carotenoid contents), and tolerant to O3 (e.g., as confirmed by the absence of visible foliar injury, the unchanged values of total carotenoids, and the detrimental effects on stomatal conductance, total chlorophylls and terpene emission only under elevated O3 concentrations). The combination of both stressors led to harsher oxidative stress. Only when evaluated together (i.e., combining the information provided by the analysis of each stress marker), ROS, MDA and photosynthetic pigments, were suitable stress markers to discriminate the differential oxidative stress induced by drought and increasing O3 concentrations applied singly or in combination: (i) all these stress markers were affected under drought per se; (ii) hydrogen peroxide (H2O2) and MDA increased under O3 per se, following the gradient of O3 concentrations (H2O2: about 2- and 4-fold higher; MDA: +22 and + 91%; in 1.5 × AA_WW and 2.0 × AA_WW, respectively); (iii) joining together the ROS it was possible to report harsher effects under 2.0 × AA_WS and 1.5 × AA_WS (both anion superoxide and H2O2 increased) than under 2.0 × AA_WW (only H2O2 increased); and (iv) MDA showed harsher effects under 2.0 × AA_WS than under 1.5 × AA_WS (increased by 49 and 18%, respectively). Plants activated physiological and biochemical adjustments in order to partially avoid (e.g., stomatal closure) and tolerate (e.g., increased terpene emission) the effects of drought when combined with increasing O3 concentrations, suggesting that the water use strategy (isohydric) and the sclerophyllous habit can further increase the plant tolerance to environmental constraints in the Mediterrane.

Environmental research (Online) 201

DOI: 10.1016/j.envres.2021.111615

2021, Articolo in rivista, ENG

Short-Term Effects of Forest Therapy on Mood States: A Pilot Study

Meneguzzo F., Albanese L., Antonelli M., Baraldi R., Becheri F.R., Centritto F., Donelli D., Finelli F., Firenzuoli F., Margheritini G., Maggini V., Nardini S., Regina M., Zabini F., Neri L.

Immersion in forest environments was shown to produce beneficial effects to human health, in particular psychophysical relaxation, leading to its growing recognition as a form of integrative medicine. However, limited evidence exists about the statistical significance of the effects and their association with external and environmental variables and personal characteristics. This experimental study aimed to substantiate the very concept of forest therapy by means of the analysis of the significance of its effects on the mood states of anxiety, depression, anger and confusion. Seven forest therapy sessions were performed in remote areas and a control one in an urban park, with participants allowed to attend only one session, resulting in 162 psychological self-assessment questionnaires administered before and after each session. Meteorological comfort, the concentration of volatile organic compounds in the forest atmosphere and environmental coherence were identified as likely important external and environmental variables. Under certain conditions, forest therapy sessions performed in remote sites were shown to outperform the control session, at least for anxiety, anger and confusion. A quantitative analysis of the association of the outcomes with personal sociodemographic characteristics revealed that only sporting habits and age were significantly associated with the outcomes for certain psychological domains.

International journal of environmental research and public health (Online) 18 (18), pp. Article number 9509

DOI: 10.3390/ijerph18189509

2021, Articolo in rivista, ENG

Date palm responses to a chronic, realistic ozone exposure in a FACE experiment

Paoletti E.; Hoshika Y.; Arab L.; Martini S.; Cotrozzi L.; Weber D.; Ache P.; Neri L.; Baraldi R.; Pellegrini E.; Müller H.M.; Hedrich R.; Alfarraj S.; Rennenberg H.

Date palms are highly economically important species in hot arid regions, which may suffer ozone (O3) pollution equivalently to heat and water stress. However, little is known about date palm sensitivity to O3. Therefore, to identify their resistance mechanisms against elevated O3, physiological parameters (leaf gas exchange, chlorophyll fluorescence and leaf pigments) and biomass growth responses to realistic O3 exposure were tested in an isoprene-emitting date palm (Phoenix dactylifera L. cv. Nabut Saif) by a Free-Air Controlled Exposure (FACE) facility with three levels of O3 (ambient [AA, 45 ppb as 24-h average], 1.5 x AA and 2 x AA). We found a reduction of photosynthesis only at 2 x AA although some foliar traits known as early indicators of O3 stress responded already at 1.5 x AA, such as increased dark respiration, reduced leaf pigment content, reduced maximum quantum yield of PSII, inactivation of the oxygen evolving complex of PSII and reduced performance index PITOT. As a result, O3 did not affect most of the growth parameters although significant declines of root biomass occurred only at 2 x AA. The major mechanism in date palm for reducing the severity of O3 impacts was a restriction of stomatal O3 uptake due to low stomatal conductance and O3-induced stomatal closure. In addition, an increased respiration in elevated O3 may indicate an enhanced capacity of catabolizing metabolites for detoxification and repair. Interestingly, date palm produced low amounts of monoterpenes, whose emission was stimulated in 2 x AA, although isoprene emission declined at both 1.5 and 2 x AA. Our results warrant more research on a biological significance of terpenoids in plant resistance against O3 stress.

Environmental research (N. Y. N. Y., Print) 195, pp. Article number 110868

DOI: 10.1016/j.envres.2021.110868

2020, Articolo in rivista, ENG

Isoprene Emission in Darkness by a Facultative Heterotrophic Green Alga

Dani, K. G. Srikanta; Torzillo, Giuseppe; Michelozzi, Marco; Baraldi, Rita; Loreto, Francesco

Isoprene is a highly reactive biogenic volatile hydrocarbon that strongly influences atmospheric oxidation chemistry and secondary organic aerosol budget. Many phytoplanktons emit isoprene like terrestrial pants. Planktonic isoprene emission is stimulated by light and heat and is seemingly dependent on photosynthesis, as in higher plants. However, prominent isoprene-emitting phytoplanktons are known to survive also as mixotrophs and heterotrophs. Chlorella vulgaris strain G-120, a unicellular green alga capable of both photoautotrophic and heterotrophic growth, was examined for isoprene emission using GC-MS and real-time PTR-MS in light (+CO2) and in darkness (+glucose). Chlorella emitted isoprene at the same rate both as a photoautotroph under light, and as an exclusive heterotroph while feeding on exogenous glucose in complete darkness. By implication, isoprene synthesis in eukaryotic phytoplankton can be fully supported by glycolytic pathways in absence of photosynthesis, which is not the case in higher plants. Isoprene emission by chlorophyll-depleted mixotrophs and heterotrophs in darkness serves unknown functions and may contribute to anomalies in oceanic isoprene estimates.

Frontiers in plant science 11

DOI: 10.3389/fpls.2020.598786

2020, Contributo in volume, ITA

Foreste, prevenzione e cura: evidenze scientifiche

Zabini F., Meneguzzo F., Albanese L., Nocetti M., Brunetti M., Neri L., Baraldi R.

In questo capitolo sono esplorati i molti ruoli, tra cui alcuni di straordinaria importanza emersi negli ultimi anni, degli ecosistemi forestali rispetto alla stabilità e sicurezza della vita umana. Dalle pandemie al clima, passando per le minacce dirette alla sopravvivenza della nostra civiltà, le grandi foreste mondiali rappresentano un patrimonio già ampiamente saccheggiato che necessita urgentemente di protezione e di una nuova espansione. Sono prospettate soluzioni, difficili ma ineludibili, pena il rischio che quello che abbiamo vissuto con il Covid-19 possa apparire in un futuro troppo vicino come un episodio insignificante. Un paragrafo è dedicato allo straordinario significato e valore delle foreste italiane rispetto al patrimonio forestale naturale dell'Europa e oltre: la ricchezza genetica di numerose specie arboree residenti nelle nostre foreste, che deve essere preservata a tutti i costi, rappresenta la più sicura garanzia di resilienza e ricostituzione non solo rispetto a una lontanissima (per noi) nuova era glaciale, ma nell'immediato rispetto alla pressione esercitata dai cambiamenti climatici globali.

DOI: 10.26388/IBE201217

2020, Articolo in rivista, ENG

Ozone impairs the response of isoprene emission to foliar nitrogen and phosphorus in poplar

Hoshika Y., Brilli F., Baraldi R., Fares S., Carrari E., Zhang L., Badea O., Paoletti E.

Tropospheric ozone (O3) impairs physiological processes of plants while nitrogen (N) deposition may cause imbalances in soil N and other nutrients such as phosphorus (P) suggesting an increase of P demand for plants. However, the combined effect of O3, soil N and P on isoprene emission from leaves has never been tested. We therefore examined isoprene emission in leaves of Oxford poplar clone exposed to O3 (ambient, AA [35.0 nmol mol-1 as daily mean]; 1.5 × AA; 2.0 × AA), soil N (0 and 80 kg N ha-1) and soil P (0, 40 and 80 kg P ha-1) in July and September in a Free-Air Controlled Exposure (FACE) facility. We also investigated the response of isoprene emission to foliar N, P and abscisic acid (ABA) contents in September because the 2-C-methylerythritol-5-phosphate (MEP) pathway of isoprenoid biosynthesis produces ABA. We found that O3 increased isoprene emission in July, which was associated to increased dark respiration, suggesting an activation of metabolism against O3 stress as an initial response. However, O3 decreased isoprene emission in September which was associated to reduced net photosynthesis. In September, isoprene emission was positively correlated with leaf N content and negatively correlated with leaf P content in AA. However, no response of isoprene emission to foliar N and P was found in elevated O3, suggesting that the isoprene responses to foliar N and P depended on the O3 exposure levels. Isoprene emission rate in 1.5 × AA and 2.0 × AA increased with increasing leaf ABA content, indicating accelerated senescence of injured leaves to favor new leaf growth when high O3 and nutritional availability in the soil were combined. Even though foliar N and P usually act as a proxy for isoprene emission rate, the impact of recent abiotic factors such as O3 should be always considered for modeling isoprene emission under climate change.

Environmental pollution (1987) 267, pp. 115679

DOI: 10.1016/j.envpol.2020.115679

2019, Articolo in rivista, ENG

Impact of Drought and Salinity on Sweetgum Tree (Liquidambar styraciflua L.): Understanding Tree Ecophysiological Responses in the Urban Context

Rita Baraldi 1 , Arkadiusz Przybysz 2 , Osvaldo Facini 1, Lorenzo Pierdonà 1, Giulia Carriero 1, Gianpaolo Bertazza 1, Luisa Neri 1

Understanding urban tree responses to drought, salt stress, and co-occurring stresses, as well as the capability to recover afterward, is important to prevent the cited stresses' negative effects on tree performance and ecological functionality. We investigated the impact of drought and salinity, alone and in combination, on leaf water potential, gas exchange, chlorophyll a fluorescence, xanthophyll cycle pigments, and isoprene emission of the urban tree species Liquidambar styraciflua L. Generally, drought had a rapid negative impact, while the effect of salt stress was more long lasting. Both stressors significantly decreased photosynthesis, transpiration, and stomatal conductance, as well as the maximum quantum effciency of photosystem II (Fv/Fm) and the photochemical effciency of PSII (FPSII), but increased nonphotochemical quenching (NPQ). Under stress conditions, a strong negative correlation between the PSII effciency and the xanthophyll cycle pigment composition indicated a nocturnal retention of zeaxanthin and antheraxanthin in a state primed for energy dissipation. Drought and salt stress inhibited isoprene emission from leaves, although its emission was less responsive to stresses than stomatal conductance and photosynthesis. Full recovery of photosynthetic parameters took place after rewatering and washing off of excess salt, indicating that no permanent damage occurred, and suggesting downregulation rather than permanent impairment of the photosynthetic apparatus. Sweetgum trees were capable of withstanding and surviving moderate drought and salt events by activating defense mechanisms conferring tolerance to environmental stresses, without increasing the emission in the atmosphere of the highly reactive isoprene.

Forests

DOI: 10.3390/f10111032

2019, Articolo in rivista, ENG

Ozone-induced impairment of night-time stomatal closure in O3-sensitive poplar clone is affected by nitrogen but not by phosphorus enrichment

Hoshika Y., De Carlo A., Baraldi R., Neri L., Carrari E., Agathokleous E., Zhang L., Fares S., Paoletti E.

Nocturnal transpiration may be a key factor influencing water use in plants. Tropospheric ozone (O3) and availability of nutrients such as nitrogen (N) and phosphorus (P) in the soil can affect daytimewater use through stomata, but the combined effects of O3, N and P on night-time stomatal conductance (gs) are not known. We investigated the effects of O3 and soil availability of N and P on nocturnal gs and the dynamics of stomatal response after leaf severing in an O3-sensitive poplar clone (Oxford) subjected to combined treatments over a growing season in an O3 free air controlled exposure (FACE) facility. The treatments were two soil N levels (0 and 80 kg N ha-1; N0 and N80), three soil P levels (0, 40 and 80 kg P ha-1; P0, P40 and P80) and three O3 levels(ambient concentration, AA [35.0 ppb as hourly mean]; 1.5 × AA; 2.0 × AA). The analysis of stomatal dynamics after leaf severing suggested that O3 impaired stomatal closure execution. As a result, nocturnal gs was increased by 2.0 × AA O3 in August (+39%) and September (+108%). Night-time gswas correlatedwith POD0 (phytotoxic O3 dose) and increased exponentially after 40 mmol m-2 POD0. Such increase of nocturnal gswas attributed to the emission of ethylene due to 2.0 × AA O3 exposure, while foliar abscisic acid (ABA) or indole-3-acetic acid (IAA) did not affect gs at night. Interestingly, the O3-induced stomatal opening at night was limited by N treatments in August, but not limited in September. Phosphorus decreased nocturnal gs, although P did not modify the O3-induced stomatal dysfunction. The results suggest that the increased nocturnal gs may be associated with a need to improve N acquisition to cope with O3 stress.

Science of the total environment 692, pp. 713–722

DOI: 10.1016/j.scitotenv.2019.07.288

2019, Articolo in rivista, ENG

An integrated study on air mitigation potential of urban vegetation: From a multi-trait approach to modeling

Baraldi, R.; Chieco, C.; Neri, L.; Facini, O.; Rapparini, F.; Morrone, L.; Rotondi, A.; Carriero, G.

The ecosystem services provided by urban forests contribute to ameliorate air quality and human well-being in cities. An integrated approach based on direct measurements of leaf functional multi-traits and on estimation of the plant mitigation potential was used for predicting the species-specific impact on air quality of 29 species, including trees and shrubs, commonly present in the urban context. In addition, volatile organic compound (VOC) emissions and ozone forming potential (OFP) of each species were evaluated. At plant levels, pollution deposition equations and the i-Tree Eco model were applied for estimating particulate (PM10) and ozone (O-3) removal potential and for calculating carbon dioxide (CO2) storage and sequestration by the studied species. The results highlight the plant species-specific ability to capture atmospheric pollutants based on their physiological (CO2 assimilation and stomatal conductance) and morphological (stomata, trichomes, waxes and cuticular ornamentation) leaf traits. Trees with abundant trichomes, waxes and wrinkled leaf surfaces are considered more suitable for capturing pollutants. Most of the studied species are suitable for urban planning programs as they result for the majority low VOC emitters and consequently are characterized by low or moderate OFP. Annual O-3 and PM10, removal of the investigated trees species ranged from about 58-140 g plant(-1) yr(-1) and from about 17-139 g plant(-1) yr(-1), respectively. Total tree CO2 storage ranged from about 164-215 kg plant(-1) and gross annual CO2 sequestration from 11 to 20 kg plant(-1) year(-1). Liriodendron tulipifera, Celtis australis, Acer campestre and Acer platanoides, were efficient species in capturing PM10 and absorbing O-3. Prunus cerasifera, Quercus cerris, together with Celtis australis, Acer campestre and Acer platanoides, were efficient for carbon sequestration and storage. As aspected, lower potential of pollutant removal and CO2 storage and sequestration were estimated for shrubs, due to their smaller leaf area and structure.

Urban forestry & urban greening (Print) 41, pp. 127–138

DOI: 10.1016/j.ufug.2019.03.020

2019, Articolo in rivista, ENG

Chemical Ecology of Capnodis tenebrionis (L.) (Coleoptera: Buprestidae): Behavioral and Biochemical Strategies for Intraspecific and Host Interactions

Bari G.; Scala A.; Garzone V.; Salvia R.; Yalcin C.; Vernile P.; Aresta A. M.; Facini O.; Baraldi R.; Bufo S. A.; Vogel H.; de Lillo E.; Rapparini F.; Falabella P.

This study focuses on several aspects of communication strategies adopted by adults of the Mediterranean flat-headed root-borer Capnodis tenebrionis (Coleoptera: Buprestidae). Morphological studies on the structures involved in mate recognition and acceptance revealed the presence of porous areas in the pronota in both sexes. These areas were variable in shape and size, but proportionally larger in males. The presence of chaetic, basiconic, and coeloconic sensilla in the antennae of both males and females was verified. Bioassays revealed stereotyped rituals in males and the involvement of female pronotal secretions in mate recognition and acceptance. During the mating assays, the female's pronotum was covered by a biologically inert polymeric resin (DenFil (TM)), which prevented males from detecting the secretions and from completing the copulation ritual. The use of the resin allowed for the collection of chemical compounds. GC-MS analysis of the resin suggested it may be used to retain compounds from insect body surfaces and revealed sex-specific chemical profiles in the cuticles. Since adult C. tenebrionis may use volatile organic compounds (VOCs) emitted from leaves or shoots, the VOC emission profiles of apricot trees were characterized. Several volatiles related to plant-insect interactions involving fruit tree species of the Rosaceae family and buprestid beetles were identified. To improve understanding of how VOCs are perceived, candidate soluble olfactory proteins involved in chemoreception (odorant-binding proteins and chemosensory proteins) were identified using tissue and sex-specific RNA-seq data. The implications for chemical identification, physiological and ecological functions in intraspecific communication and insect-host interactions are discussed and potential applications for monitoring presented.

Frontiers in physiology 10 (604)

DOI: 10.3389/fphys.2019.00604

2019, Presentazione, ENG

"Direct measurements of CH4 emissions from illegally managed waste landfills in Giugliano (Campania, Italy): a novel approach"

D. Famulari, G. Agrillo, R. Baraldi, B. Brusasca, P. Carlucci, G. Carriero, P. Ciccioli, A. Chirico, S. Di Lonardo, P. Di Tommasi, A. Esposito, O. Facini, C. Ferrara, S. Finardi, D. Gasbarra, B. Gioli, D. Guadagnuolo, V. Magliulo, A. Manco, L. Neri, D. Piscitelli, A. Riccio, I. Russo, M. Tosca, P. Toscano, L. Vitale, A. Zaldei

Presentation of the direct measurements of CH4 emissions from illegally managed waste landfills in Giugliano (Campania, Italy)

8th International Symposium on Non-CO2 Greenhouse Gases: Global Challenges and Local Solutions, Amsterdam, The Netherlands, June 12-14, 2019

2019, Articolo in rivista, ENG

Can nutrient fertilization mitigate the effects of ozone exposure on an ozone-sensitive poplar clone?

Podda A., Pisuttu C., Hoshika Y., Pellegrini E., Carrari E., Lorenzini G., Nali C., Cotrozzi L., Zhang L., Baraldi R., Neri L., Paoletti E.

We tested the independent and interactive effects of nitrogen (N; 0 and 80 kg ha(-1)), phosphorus (P; 0, 40 and 80 kg ha(-1)), and ozone (O-3) application/exposure [ambient concentration (AA), 1.5 x AA and 2.0 x AA] for five consecutive months on biochemical traits of the O-3-sensitive Oxford poplar clone. Plants exposed to O-3 showed visible injury and an alteration of membrane integrity, as confirmed by the malondialdehyde byproduct accumulation (+3 and + 17% under 1.5 x AA and 2.0 x AA conditions, in comparison to AA). This was probably due to O-3-induced oxidative damage, as documented by the production of superoxide anion radical (O-2(.-), +27 and -E 63%, respectively). Ozone per se, independently from the concentrations, induced multiple signals (e.g., alteration of cellular redox state, increase of abscisic acickindole-3-acetic acid ratio and reduction of proline content) that might be part of premature leaf senescence processes. By contrast, nutrient fertilization (both N and P) reduced reactive oxygen species accumulation (as confirmed by the decreased O-2(.-) and hydrogen peroxide content), resulting in enhanced membrane stability. This was probably due to the simultaneous involvement of antioxidant compounds (e.g., carotenoids, ascorbate and glutathione) and osmoprotectants (e.g., proline) that regulate the detoxification processes of coping with oxidative stress by redwing the O-3 sensitivity of Oxford clone. These mitigation effects were effective only under AA and 1.5 x AA conditions. Nitrogen and P supply activated a free radical scavenging system that was not able to delay leaf senescence and mitigate the adverse effects of a general peroxidation due to the highest 03 concentrations. (C) 2018 Elsevier B.V. All rights reserved.

Science of the total environment 657, pp. 340–350

DOI: 10.1016/j.scitotenv.2018.11.459

2019, Articolo in rivista, ENG

Root colonization by Pseudomonas chlororaphis primes tomato (Lycopersicum esculentum) plants for enhanced tolerance to water stress

Brilli F., Pollastri S., Raio A., Baraldi R., Neri L., Bartolini P., Podda A., Loreto F., Maserti B., Balestrini R.

Previous research demonstrated that Pseudomonas chlororaphis subsp. aureofaciens strain M71, a plant growth promoting bacterium (PGPB), exerts beneficial effects on plant metabolism and primes tolerance mechanisms against biotic stresses in tomatoes. We designed an experiment to assess whether root colonization with P. chlororaphis is also able to improve tolerance to water stress in tomatoes. Our results show that inoculation with P. chlororaphis stimulates the antioxidant activity of well-watered tomatoes while maintaining a steady-state level of reactive oxygen species (ROS), increases the expression of genes encoding for the biosynthesis of leaf terpenes, stimulates the production of both the phytohormones ABA and IAA, in turn affecting plant shape (number of leaves) and height (length of internodes), without altering photosynthesis. Upon exposure to mild water stress conditions, an improved antioxidant activity in tomatoes 'primed' by P. chlororaphis inoculation limited the accumulation of reactive oxygen species (ROS) in leaves and thus enhanced tolerance, also through increase of the (osmolyte) proline content. Moreover, P. chlororaphis inoculation further enhanced the ABA level in leaves of water-stressed tomatoes allowing a more efficient modulation of stomatal closure that resulted in an improved water use efficiency (WUE) and biomass accumulation.

Journal of plant physiology (Print) 232, pp. 82–93

DOI: 10.1016/j.jplph.2018.10.029

2019, Articolo in rivista, ENG

Ecophysiological and micromorphological characterization of green roof vegetation for urban mitigation

Baraldi R., Neri L., Costa F., Facini O., Rapparini F., Carriero G.

Urban greening helps to improve environment quality and human health thanks to the ability of plants to absorb greenhouse gases and remove significant amounts of air pollutants. Green roofs, providing vegetated surfaces in dense cities, can help to mitigate the negative effects of urban pollution. The present study aimed to evaluate the potential ability of fifteen green roof species (Lonicera pileata, Satureja repandens, Hypericum moserianum, Erigeron karvinskianus, Solidago praecox, Rudbeckia sullivantii 'Goldsturm', Filipendula purpurea, Filipendula vulgaris 'Kahome', Gaura lindheimeri, Campanula persicifolia, Veronica longifolia, Sedum spectabile, Origanum vulgare, Salvia nemorosa, and Achillea millefolium) to mitigate carbon dioxide (CO2) and urban pollutant concentration analysing the leaf physiological traits (gas exchange) and morphological structures (stomata, trichomes, epicuticular waxes and cuticular ornamentation) involved in pollutant removal. Furthermore, considering the important role of biogenic volatile organic compounds (BVOC) on the photochemical reactivity of the atmosphere, their species-specific emission was also assessed together with the derived ozone forming potential (OFP). Our results suggested that the potential mitigation capacity based on the investigated traits of the shrubs and herbaceous species was species-specific. The very low potential of all the tested species to form ozone indicated their suitability for urban planning programs.

Urban forestry & urban greening (Internet) 37, pp. 24–32

DOI: 10.1016/j.ufug.2018.03.002

2018, Rapporto di ricerca (Research report), ITA

Servizio di sviluppo ed implementazione di un sistema integrato finalizzato alla valutazione degli impatti sulla qualità dell'aria, per la gestione degli interventi previsti nel Piano Regolatore del Porto di Livorno (PRP)

Rita Baraldi; Federico Carotenuto; Osvaldo Facini; Beniamino Gioli; Giovanni Gualtieri; Francesca Martelli; Carla Mazziotti; Laura Pellegrino; Graziella Rossini; Piero Toscano; Marin Tudoroiu; Carolina Vagnoli; Alessandro Zaldei

Il lavoro si inquadra nell'ambito degli adempimenti connessi alle prescrizioni della procedura di Valutazione Ambientale Strategica (VAS) del nuovo Piano Regolatore Portuale (PRP) attuato dall'Autorità Portuale di Livorno. Tra i suddetti adempimenti rientra lo sviluppo di un modello integrato che fornisca dinamicamente informazioni sulla qualità dell'aria, comprendendo non solo gli effetti del singolo progetto, ma anche l'effetto combinato delle singole azioni precedentemente attuate; quest'ultimo aspetto ha preso in esame sia l'ambito di attuazione del PRP, che altre strategie di pianificazione ed attuazione sviluppatesi nel frattempo (Piano Regolatore Generale, piani di settore quali la mobilità, energetico, di risanamento atmosferico, acustico, etc.)

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Baraldi Rita

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