Prospective life cycle assessment models were developed and applied at the laboratory and industrial scale with the aim to evaluate the environmental burdens associated with the LimoFish process used to produce the fish oil "AnchoiOil", the new organic fertilizer "AnchoisFert" or biogas (by means of anaerobic digestion) after treatment of anchovy fillet leftovers (AnLeft) with agro-solvent d-limonene. Potential impacts for climate change and freshwater eutrophication were estimated at 29.1 kg CO2 eq/kg AnLeft and 1.7E- 07 kg PO4 eq/kg AnLeft at laboratory scale, and at 1.5 kg CO2 eq/kg AnLeft and 2.2E- 07 kg PO4 eq/kg AnLeft at industrial scale. Electricity consumption is the main contributor to the environmental impact of the process and plays a significant role in the production of d-limonene, for which cold pressing extraction would reduce the related impacts by ~ 70 %. The use of the solid by-product as organic fertilizer or input to anaerobic digestion would provide additional environmental benefits to the process. The LimoFish process is a successful example of a low impacting strategy to reduce the demand for natural resources and maximize the application of the circular economy principles in the fishing industry.

Aiming to identify and assess the amount of fat-soluble vitamins in anchovy oil extracted with d-limonene from anchovy fillet leftovers via the UHPLC-HESI-MS advanced mass spectrometry analytical technique, this study shows that only vitamin D is present in anchovy by-product oil. The sum of the quantities of the three isomers of vitamin D amounts to 0.0815 ?g vitamin D per g oil, namely a 81.5 ?g/kg content, in good agreement with the typical amounts of vitamin D in fish oils (ranging from 18 to 350 ?g/kg).

Fish oil rich in polyunsaturated omega-3 fatty acids is extracted in high yield from anchovy filleting waste using d-limonene as green biosolvent in a simple solid-liquid extraction performed by mechanically stirring and maceration followed by limonene removal via evaporation under reduced pressure. As limonene is renewably obtained from waste orange peel, this method closes the materials cycle and establishes a circular economy process to obtain high quality fish oil from biowaste available worldwide in several million t/year amount. Significant economic, social and environmental benefits are anticipated.

Fisheries products found in the landing places or markets usually represent a fraction of what has been originally taken out from the sea by fishermen. A more or less variable amount of the gross (or overall or initial) catch, in fact, is returned either dead or alive to the sea for multiple reasons. This phenomenon, generally known as 'the discard problem', has raised more and more global concern especially in the last decades since introducing more selectivity gears and acquiring more reliable catch data (also about the survival rate of the discarded specimens) are considered basic requirements in the current management plans for recovering and rebuilding fish stocks. To this concern, the current Common Fisheries Policy aims to reduce the discard rate in the European waters through the introduction of a landing obligation (better 'discard ban') according to which all the specimens of the 'target' stock caught must be landed. This chapter focuses on the characterization of the catch returned to the sea 'potential' available to be landed after the introduction of landing obligation, and the possible use as a potential food source in aquaculture (the direct use for human consumption is in fact prohibited).

Nowadays, dietary guidelines acknowledge the therapeutic role of omega-3 polyunsaturated fatty acids, as the most important class of fatty acids, against different human diseases. During the last two decades, the average level of consumption of omega-3 polyunsaturated fatty acids has increased from 0.1 to 0.2 g per day. Omega-3 polyunsaturated fatty acids are a group of long-chain polyunsaturated fatty acids which are identified in different foods such as fatty fish, shellfish, and vegetable oils. A growing body of epidemiological and experimental evidence supports the anticancer effects of omega-3 polyunsaturated fatty acids, which led to the identification of their molecular targets in several cancer models. The present review focuses on the basic evidence supporting the potential applications of omega-3 polyunsaturated fatty acids in cancer therapy.