Articolo in rivista, 2006, ENG, 10.1186/1742-4933-3-7

Electrophoresis of proteins and DNA on horizontal sodium dodecyl sulfate polyacrylamide gels

Vincenzo Izzo 1; Maria A Costa 1; Renata Di Fiore 1; Giovanni Duro 1; Daniele Bellavia 2; Eleonora Cascone 2; Paolo Colombo 1; Maria C Gioviale 3 and Rainer Barbieri2

1 Istituto di Biomedicina e Immunologia Molecolare, C.N.R., Via U. La Malfa, 153, 90153 Palermo, Italy. 2 Dipartimento di BiologiaCellulare e dello Sviluppo - Università di Palermo, V.le delle Scienze, 90128 Palermo, Italy 3 Dipartimento GEN.UR.TO. - Università di Palermo, Italy

An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels of different dimensions for different purposes, is described. Preparation of such gels is as easy and rapid as agarose gel preparation, and polymerized polyacrylamide gels are used to fractionate proteins or small DNA fragments using a common horizontal electrophoretic tank. This apparatus was used to electrophoretically fractionate proteins or DNA for immuno-blot analyses, particularirly in the study of the allergenic response to Parietaria judaica pollen in senescence, for Southern-blot hybridizations and in the study of DNA polymorphisms.

Immunity & ageing 3 (7), pp. 1–5

Keywords

CNR authors

Colombo Paolo, Costa Maria Assunta, Izzo Vincenzo, Duro Giovanni

CNR institutes

IBIM – Istituto di biomedicina e di immunologia molecolare "Alberto Monroy"

ID: 12241

Year: 2006

Type: Articolo in rivista

Creation: 2009-06-16 00:00:00.000

Last update: 2012-05-03 10:27:22.000

External links

OAI-PMH: Dublin Core

OAI-PMH: Mods

OAI-PMH: RDF

DOI: 10.1186/1742-4933-3-7

External IDs

CNR OAI-PMH: oai:it.cnr:prodotti:12241

DOI: 10.1186/1742-4933-3-7