Articolo in rivista, 2006, ENG, 10.1186/1742-4933-3-7
Vincenzo Izzo 1; Maria A Costa 1; Renata Di Fiore 1; Giovanni Duro 1; Daniele Bellavia 2; Eleonora Cascone 2; Paolo Colombo 1; Maria C Gioviale 3 and Rainer Barbieri2
1 Istituto di Biomedicina e Immunologia Molecolare, C.N.R., Via U. La Malfa, 153, 90153 Palermo, Italy. 2 Dipartimento di BiologiaCellulare e dello Sviluppo - Università di Palermo, V.le delle Scienze, 90128 Palermo, Italy 3 Dipartimento GEN.UR.TO. - Università di Palermo, Italy
An inexpensive Plexiglas apparatus which allows a simple and rapid preparation of horizontal polyacrylamide gels of different dimensions for different purposes, is described. Preparation of such gels is as easy and rapid as agarose gel preparation, and polymerized polyacrylamide gels are used to fractionate proteins or small DNA fragments using a common horizontal electrophoretic tank. This apparatus was used to electrophoretically fractionate proteins or DNA for immuno-blot analyses, particularirly in the study of the allergenic response to Parietaria judaica pollen in senescence, for Southern-blot hybridizations and in the study of DNA polymorphisms.
Immunity & ageing 3 (7), pp. 1–5
Colombo Paolo, Costa Maria Assunta, Izzo Vincenzo, Duro Giovanni
IBIM – Istituto di biomedicina e di immunologia molecolare "Alberto Monroy"
ID: 12241
Year: 2006
Type: Articolo in rivista
Creation: 2009-06-16 00:00:00.000
Last update: 2012-05-03 10:27:22.000
External IDs
CNR OAI-PMH: oai:it.cnr:prodotti:12241
DOI: 10.1186/1742-4933-3-7