CNR ExploRA

Articolo in rivista, 2017, ENG, 10.1364/OE.25.017746

Digital holography as 3D tracking tool for assessing acoustophoretic particle manipulation

Cacace T, Paturzo M, Memmolo P, Vassalli M, Ferraro P, Fraldi M, Mensitieri G

National Research Council of Italy, Institute of Applied Sciences and Intelligent Systems 'E. Caianiello', Via Campi Flegrei 34, Pozzuoli, Naples, Italy; Department of Mathematics and Physics, University of Campania "L. Vanvitelli", Viale Lincoln 5, 81100, Caserta, Italy; CNR - Institute for Microelectronics and Microsystems, Unit of Naples, via P. Castellino 111 80131 Naples, Italy; National Research Council of Italy, Institute of Biophysics, Via De Marini 6, 16149 Genova, Italy; Department of Structures for Engineering and Architecture, University of Naples Federico II, Piazzale Tecchio 80, 80125 Naples, Italy; Department of Chemical, Materials and Production Engineering University of Naples Federico II, Piazzale Tecchio 80, 80125 Naples, Italy

The integration of digital holography (DH) imaging and the acoustic manipulation of micro-particles in a microfluidic environment is investigated. The ability of DH to provide efficient 3D tracking of particles inside a microfluidic channel is exploited to measure the position of multiple objects moving under the effect of stationary ultrasound pressure fields. The axial displacement provides a direct verification of the numerically computed positions of the standing wave's node, while the particle's transversal movement highlights the presence of nodes in the planar direction. Moreover, DH is used to follow the aggregation dynamics of trapped spheres in such nodes by using aggregation rate metrics.

Optics express 25 (15), pp. 17746–17752