Ganino T., Silvanini A., Benelli C., Beghè D., Fabbri A.
Ganino T., Silvanini A., Beghè D., Fabbri A.: Dipartimento di Biologia Evolutiva e Funzionale, Sezione Biologia Vegetale, Università di Parma, via Usberti 11/a, 43100 Parma.
Synthetic seed technology has been recently applied to in vitro grown plant explants, such as shoot tips and nodal segments. It is one of the most important and advance applications of in vitro culture. Moreover, synthetic seeds are utilised for innovative cryopreservation procedures, such as encapsulation-dehydration and encapsulation-vitrification, to preserve for long-term germplasm. This study was aimed to the development and application of the synthetic seeds technique in micropropagation, carrying out a protocol for encapsulation and storage of the rootstock Kober 5BB. In vitro grown Kober 5BB explants, shoot tips and nodal segments, were encapsulated into an artificial endosperm. Different sucrose concentrations (0, 10, 30 e 60 g l-1) were added in alginate beads, to evaluate its effects on germination. For germination the synthetic seeds were maintained on semi-solid MS medium, at 23°C with 16 h of photoperiod. Germination percentage and germination time were recorded. To evaluate in vitro conservation of encapsulated explants of the grape rootstock, synthetic seeds with shoot tips and nodal segments were kept at 4°C under light and dark conditions, for 3, 6 and 9 months. Germination percentage was evaluated at the end of each storage period, after transferring in a proliferation substrate (MS medium, added with 5 ¼M BA) under standard growth conditions.
coltura in vitro, incapsulazione, conservazione in condizioni di crescita rallentata
IVALSA – Istituto per la valorizzazione del legno e delle specie arboree
ID: 73812
Year: 2009
Type: Articolo in rivista
Creation: 2009-06-16 00:00:00.000
Last update: 2009-06-16 00:00:00.000
CNR authors
External IDs
CNR OAI-PMH: oai:it.cnr:prodotti:73812